& Sasaki, A. In this study, we demonstrate that S. purpurea extracts can inhibit the replication of HSV-1 by two distinct mechanisms of action. j. to gtts xx. Kannan, L., Kumar, A., Kumar, A. et al. The cell monolayers were photographed at 24h.p.i. Sarracenia purpurea is an evergreen Perennial growing to 0.3 m (1ft) by 0.3 m (1ft in) at a medium rate. 1B, a dose dependent reduction in plaque formation was observed with a 50% reduction in plaques observable at approximately 30g/ml. To quantitate this anti-HSV-1 effect, a plaque reduction assay was performed. Kress H Henriette's Herbal Homepage website. The work described characterizes the antipoxvirus activity associated with this botanical extract against vaccinia virus, monkeypox virus and variola . Infected cells were washed twice with warm media and then given fresh media containing S. purpurea. Seek emergency medical attention or call the Poison Help line at 1-800-222-1222. 1862;80:615616. Since S. purpurea extracts inhibited HSV-1 replication when added at the time of infection and the reduction in viral titers were below that of input virus (Fig. S. purpurea reduced HSV-1 ICP4, ICP8, and gC protein levels in a time dependent manner. IC50 were calculated as the dose of the extract required to inhibit viral plaque formation by 50%. The side effects of pitcher plant taken by mouth are not known. Clinical trials demonstrated that this drug reduced the healing time of herpes labialis lesions by only 17.5h on average. Sarapin is a grandfathered FDA-approved prescription product. Vero cells were mock infected or infected with HSV-1 at a MOI=5 in the presence or absence of S. purpurea (40g/ml) added at 0, 1, 2, 4, and 6h.p.i. and transmitted securely. J. Ethnopharmacol. Vero cells (ATCC CCL-81) were maintained with Minimal Essential Media (Cellgro) supplemented with 10% heat inactivated fetal bovine serum (Hyclone) and 1% AntibioticAntimycotic (ThermoFisher). Statistical analysis was performed using a paired t-test. Montgomery, R. I., Warner, M. S., Lum, B. J. PubMed 75, 12111222 (1994). Ho, D. Y. Google Scholar. Spear, P. G., Shieh, M. T., Herold, B. C., WuDunn, D. & Koshy, T. I. Heparan sulfate glycosaminoglycans as primary cell surface receptors for herpes simplex virus. To test for this, Vero cells were infected with HSV-1, treated with S. purpurea extracts at 0, 1, 2, 4, and 6h.p.i., followed by purification of the RNA at 8h.p.i. Sarracenia purpurea, the purple pitcher plant, northern pitcher plant, turtle socks, or side-saddle flower, . Hattori, T., Ikematsu, S., Koito, A., Matsushita, S. & Maeda, Y. Be sure to follow relevant directions on product labels and consult your pharmacist or physician or other healthcare professional before using. Abubakar IB, Kankara SS, Malami I, Danjuma JB, Muhammad YZ, Yahaya H, Singh D, Usman UJ, Ukwuani-Kwaja AN, Muhammad A, Ahmed SJ, Folami SO, Falana MB, Nurudeen QO. Competing Interests: Yvan Rochon, an author on the submitted manuscript, is owner and operator of Herbal Vitality, Inc. and total RNA was isolated. Partridge, M. & Poswillo, D. E. Topical carbenoxolone sodium in the management of herpes simplex infection. Careers. Levels of protein expression on the Western blots were quantified using ImageQuant software. Our previous studies demonstrated that S. purpurea extracts could inhibit the accumulation of HSV-1 proteins suggesting an inhibition of viral replication33. 1996-2023 RxList, Inc. All rights reserved. Anti-herpes virus activity of the carnivorous botanical, https://doi.org/10.1038/s41598-020-76151-w. Get the most important science stories of the day, free in your inbox. Review of current and potential clinical uses. Figure 3. Gene expression levels were measured by real-time PCR using gene specific primers for ICP4 (GCGACGACGACGAGAAC and CGAGTACAGCACCACCAC), ICP8 (GGACTACGGCGCGATAAA and CGTGAGGGTGTTGATGAAGTA), gC (GAGGTCCTGACGAACATCAC and GCCCGGTGACAGAATACAA) and actin. 105, 5563 (2006). CC50 was calculated as the dose of the extract that led to 50% cell cytotoxicity. Chatis, P. A., Miller, C. H., Shrager, L. E. & Crumpaker, C. S. Successful treatment with foscarnet of an acyclovir resistant mucocutaneus infection with herpes simplex virus in a patient with acquired immunodeficiency syndrome. These results, along with our previous study, support that the S. purpurea extract contains bioactive anti-herpes components with limited or no cell toxicity at the doses tested34. CAS Google Scholar. 3 were done with the extraction vehicle alone (50% ethanol/10% glycerin) and did not demonstrate any notable effect on viral attachment (data not shown). & Bryson, H. M. A reappraisal of its antiviral activity, pharmacokinetic properties and therapeutic use in immunocompromised patients with viral infections. Samples were separated on 10% polyacrylamide gels, transferred to nitrocellulose membrane in blotting transfer buffer (10mM CAPS buffer pH 11.0, 20% methanol) and blocked with 25mM Tris, pH 7.5, 137mM NaCl, 2.5mM KCl, 0.025% Tween, 5% powdered milk. Oral. When S. purpurea extracts were added at 0 and 0.5h.p.i., no detectable virus was present after the 24-h growth period. Antimicrob. The easiest way to lookup drug information, identify pills, check interactions and set up your own personal medication records. Free-virus treatment was performed using 200pfu of HSV-1 KOS treated with increasing concentrations of S. purpurea and incubation for 1h at room temperature. Conventional treatment for HSV-1 infection includes pharmaceutical drugs, such as acyclovir and docosonal, which are efficacious but maintain the potential for the development of viral drug resistance. Physician's Desk Reference. To examine this further, free HSV-1 virions were incubated with the extract, followed by washing of the virus and subsequent infection. Google Scholar. The leaf and root are used as medicine. Noormohamed, F. H., Youle, M. S., Higgs, C. J., Martin-Munley, S. & Gazzard, B. G. Pharmacokinetics and absolute bioavailability of oral foscarnet in human immunodeficiency virus-seropositive patients. Repeat at greater intervals as condition subsides. Statistical analysis was performed using a paired t-test. Leduc, C., Coonishish, J., Haddad, P. & Cuerrier, A. These results may suggest a common target between poxvirus and HSV-1 viral gene expression which is being inhibited by the S. purpurea extract. Eight to twenty-four inch perennial with red-veined leaves. Sarapin is a grandfathered FDA-approved prescription product. For the late protein, gC, treatment with the extract through 6h.p.i. Parker S, Chen NG, Foster S, Hartzler H, Hembrador E, Hruby D, Jordan R, Lanier R, Painter G, Painter W, Sagartz JE, Schriewer J, Mark Buller R. Antiviral Res. Veja como este site usa. No significant viral plaque inhibition or cell toxicity was observed with the vehicle (50% ethanol/10% glycerin) alone over the dose range tested (Fig. Traditional medicinal plants used for treating emerging and re-emerging viral diseases in northern Nigeria. significantly reduced the level of ICP8 (Fig. Cells were incubated at 37C, with 5% CO2 in a humidified chamber. Herpes simplex virus latency: Molecular aspects. The purple pitcher plant is grown as an ornamental plant; it is well suited for cool greenhouses, sheltered outdoor spaces, bog gardens and damp woodland areas. At this time, a botanical preparation, derived from the carnivorous plant Sarracenia purpurea, was proclaimed as being a successful therapy for smallpox infections. Natl. Error bars indicate the standard deviation from three separate trials. CAS In the current study, we demonstrate that S. purpurea extracts can inhibit the replication of HSV-1 through two distinct mechanisms of action. There is much scepticism on herbal medicine but what our results illustrate conclusively is that this herb is able to kill the virus and we can actually demonstrate how it kills the virus, says Langland. (D) Vero cells were treated with increasing concentrations of S. purpurea extract or vehicle and cell viability measured at 24h post-treatment and the results graphed. Selected plant species from the Cree pharmacopoeia of northern Quebec possess anti-diabetic potential. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in It is hardy to UK zone 3. . Epub 2021 Nov 27. Anti-herpes simplex virus activities of monogalactosyl diglyceride and digalactosyl diglyceride from Clinacanthus nutans, a traditional Thai herbal medicine. Sarapin. You're not signed in. When the extract was added to viral infected cells up to 6h.p.i, viral replication was inhibited (Fig. (detailed description of each of the ratings). Thyagarajan, S. P., Subramanian, S., Thirunalasundari, T., Venkateswaran, P. S. & Blumberg, B. S. Effect of Phyllanthus amarus on chronic carriers of hepatitis B virus. When the extract was added at 0 or 1h.p.i., a significant reduction in the level of the immediate early protein, ICP4, was observed (Fig. http://www.henriettesherbal.com/eclectic/spec-med/sarracenia.html, R01 AI095394/AI/NIAID NIH HHS/United States. ISSN 2045-2322 (online). Manufacturer Information. This agrees with our previous studies on the effects of S. purpurea on poxviruses34. CAS Our lab has previously demonstrated the ability of S. purpurea extracts to inhibit poxvirus replication, with broad spectrum activity towards other viruses including HSV-133,34. document.write(new Date().getFullYear()); In conclusion, the S. purpurea extract inhibited the replication of HSV-1 by two distinct mechanisms of action. At this time there is not enough scientific information to determine an appropriate range of doses for pitcher plant. Read our privacy policy. & Naji, M. A. The effectiveness of these drugs, however, are limited in immune-suppressed patients, resulting in increased likelihood of the virus to develop drug resistance9,10. American Medicinal Plants: An Illustrated and Descriptive Guide to the American Plants Used as Homeopathic Remedies: Their History, Preparation, Chemistry and Physiological Effect, (New York and Philadelphia), Clarke JH. 27, 308 (1996). Looker, K. J. et al. Article 4A,B). Morrison, S. A., Li, H., Webster, D., Johnson, J. Medicinal plants contain an abundance of natural compounds and have been used traditionally throughout history in many countries to treat viral infections16,17,18,19,20,21. (A) For the viral attachment assay, Vero cells were infected with 200 pfu HSV-1 in the presence of 0, 10, 20, 40, or 60g/ml S. purpurea extract and incubated on ice for 2h. The cell monolayers were washed three times with cold media, followed by the addition of warm media and incubation for 3days. Lancet 80, 430431 (1862). Chen, T. et al. At this time, a botanical preparation, derived from the carnivorous plant Sarracenia purpurea, was proclaimed as being a successful therapy for smallpox infections. Can. Docosanol, a saturated fatty alcohol, is thought to inhibit viral replication by inhibiting the fusion of the human host cell with the viral envelope of HSV-1. Epub 2021 Jun 29. Notably, treatment with the extraction vehicle alone had no effect on viral protein synthesis (data not shown). (B) For free virus pre-treatment, 200 pfu of purified HSV-1 virions were treated with 0, 10, 20, 40, or 60g/ml S. purpurea extract and incubated at room temperature for 1h. After incubation the samples were centrifuged at 20,000g for 1h to pellet the virus. Alone had no effect on viral protein synthesis ( data not shown ) pharmacist or or... Was inhibited ( Fig & Poswillo, D., Johnson, J traditionally throughout history in many to. Were calculated as the dose of the extract required to inhibit viral plaque formation was with. For the late protein, gC, treatment with the extract that led to 50 % cytotoxicity. The management of herpes labialis lesions by only 17.5h on average is being inhibited by the S. purpurea poxviruses34... And therapeutic use in immunocompromised patients with viral infections viral replication was inhibited ( Fig HSV-1 KOS treated increasing! 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